Endoglucanase gene of M42 aminopeptidase/endoglucanase family from thermophilic Bacillus sp. PW1 and PW2 isolated from Tattapani hot spring, Himachal Pradesh, India
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چکیده
منابع مشابه
Cloning, expression, purification and characterization of lipase from Bacillus licheniformis, isolated from hot spring of Himachal Pradesh, India.
In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusion bodies demonstrated ~22 kDa protein band on 12 % SDS-PAGE. It exhibited specific activity of 0....
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background and objectives: thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. materials and methods: in this study, thermophilic bacteria were isolated from hot spring of tarabalo, india. a bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16s rrna gene...
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Studies on lipase production were carried out with a bacterial strain (Bacillus sp LBN 2) isolated from soil sample of hotspring of Arunachal Pradesh, India. The cells were cultivated in a mineral medium with maximum production at 1% groundnut oil. The optimum temperature and initial medium pH for lipase production by the organism were 50(0)C and 9.0 respectively. The molecular mass was found t...
متن کاملIsolation and characterization of a thermophilic Bacillus sp. with protease activity isolated from hot spring of Tarabalo, Odisha, India
BACKGROUND AND OBJECTIVES Thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. MATERIALS AND METHODS In this study, thermophilic bacteria were isolated from hot spring of Tarabalo, India. A bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16S rRNA gene...
متن کاملAnoxybacillus suryakundensis sp. nov, a Moderately Thermophilic, Alkalitolerant Bacterium Isolated from Hot Spring at Jharkhand, India
Four closely related facultative anaerobe, moderately thermophilic, Gram positive rods (JS1(T), JS5, JS11, and JS15) were isolated from sediment samples from a hot spring at Suryakund, Jharkhand, India. Colonies were pale yellow, rough surface with uneven edges on TSA after 72 h incubation. Heterotrophic growth was observed at 40-60°C and pH 5.5-11.5; optimum growth occurred at 55°C and pH 7.5....
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2019
ISSN: 2090-5920
DOI: 10.1186/s43141-019-0001-8